CTFReconstructed#

class CTFReconstructed(defocus=None, angles=None, opening_axis=2, tilt_axis=0, sampling_rate=1, acceleration_voltage=300, spherical_aberration=27000000.0, amplitude_contrast=0.07, phase_shift=0, astigmatism_angle=0, defocus_delta=0, correction_mode='phase-flip', wiener_falloff=None)[source]#

Bases: CTF

Generate CTF filter for reconstructions.

Attributes

`CTFReconstructed.acceleration_voltage`	The acceleration voltage in kV, defaults to 300
`CTFReconstructed.amplitude_contrast`	The amplitude contrast, defaults to 0.07.
`CTFReconstructed.angles`	The tilt angles in degrees.
`CTFReconstructed.astigmatism_angle`	The astigmatism angle in radians, defaults to 0.
`CTFReconstructed.correction_mode`	CTF correction mode: 'raw', 'wiener', 'phase-flip', 'phase-flip-weighted'.
`CTFReconstructed.defocus`	The mean defocus value in Ångstrom (positive is underfocus).
`CTFReconstructed.defocus_delta`	The defocus difference (half-delta) in Ångstrom, defaults to 0.
`CTFReconstructed.opening_axis`	The microscope projection axis, defaults to 2 (z).
`CTFReconstructed.phase_shift`	The phase shift in radians, defaults to 0.
`CTFReconstructed.sampling_rate`	The sampling rate, defaults to 1 Ångstrom / voxel.
`CTFReconstructed.spherical_aberration`	The spherical aberration, defaults to 2.7e7 (in Angstrom).
`CTFReconstructed.tilt_axis`	The axis along which the tilt is applied, defaults to 0 (x).
`CTFReconstructed.wiener_falloff`	B-factor style falloff for Wiener filter (Å²).

Methods

`CTFReconstructed.__call__`([return_real_fourier])	This method provides the standard interface for creating of composable filter masks.
`CTFReconstructed.from_file`(filename, **kwargs)	Initialize `CTF` from file.