CTFReconstructed#

class CTFReconstructed(defocus_x=None, angles=None, opening_axis=2, tilt_axis=0, sampling_rate=1, acceleration_voltage=300000.0, spherical_aberration=27000000.0, amplitude_contrast=0.07, phase_shift=0, defocus_angle=0, defocus_y=None, flip_phase=True)[source]#

Bases: CTF

Generate CTF filter for reconstructions.

Attributes

CTFReconstructed.acceleration_voltage

The acceleration voltage in Volts, defaults to 300e3.

CTFReconstructed.amplitude_contrast

The amplitude contrast, defaults to 0.07.

CTFReconstructed.angles

The tilt angles in degrees.

CTFReconstructed.defocus_angle

The defocus angle in radians, defaults to 0.

CTFReconstructed.defocus_x

The defocus in x direction (in units of sampling rate).

CTFReconstructed.defocus_y

The defocus value in y direction, defaults to None (in units of sampling rate).

CTFReconstructed.flip_phase

Whether the returned CTF should be phase-flipped, defaults to True.

CTFReconstructed.opening_axis

The microscope projection axis, defaults to 2 (z).

CTFReconstructed.phase_shift

The phase shift in radians, defaults to 0.

CTFReconstructed.sampling_rate

The sampling rate, defaults to 1 Ångstrom / voxel.

CTFReconstructed.spherical_aberration

The spherical aberration, defaults to 2.7e7 (in units of sampling rate).

CTFReconstructed.tilt_axis

The axis along which the tilt is applied, defaults to 0 (x).

Methods

CTFReconstructed.__call__([return_real_fourier])

This method provides the standard interface for creating of composable filter masks.

CTFReconstructed.from_file(filename, **kwargs)

Initialize CTF from file.